層粘連蛋白(LN)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法)
ELISA Kit DIY Materials for Laminin (LN)
LAM
- 編號KSA082Mu01
- 物種Mus musculus (Mouse，小鼠)相同的名稱，不同的物種。
- 試劑內(nèi)容包被抗體、檢測抗體、標準品、HRP標記的鏈霉親和素、TMB底物、96孔板
- 可檢測樣本Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
- 可適用原理雙抗夾心酶聯(lián)免疫吸附試驗法測抗原
- 可檢測范圍0.781-50ng/mL
- 可適靈敏度0.28ng/mL.
- 應用Main materials for "Do It (ELISA Kit) Yourself".
- 下載英文說明書 中文說明書
- 規(guī)格96T*596T*1096T*2096T*5096T*100
- 價格¥ 6221¥ 10368¥ 18662¥ 36288¥ 62208
- 欲了解實際交易價格和更多情況，請與當?shù)亟?jīng)銷商聯(lián)系!
產(chǎn)品包裝(模擬)
產(chǎn)品包裝(模擬)
通過ISO 9001、ISO 13485質(zhì)量體系認證
特異性
試劑盒中的抗體對層粘連蛋白(LN)具有高靈敏度和高特異性，經(jīng)檢測與其它相似物質(zhì)無明顯交叉反應。
用法
1.用100μL每孔的工作濃度的包被抗體包被96孔板，4度孵育過夜或者37度孵育2小時；
2.洗板1次；
3.用200μL每孔的工作濃度的封閉液,37度孵育1.5小時；
4.洗板1次。酶標板即可用于ELISA實驗，后續(xù)使用方法與ELISA同。
儲存
抗體，標準品和HRP標記的鏈霉親和素在-20度保存，TMB需在4度保存，96孔板在室溫保存。所有組分保質(zhì)期為1年，試劑一旦開封，在4度保存時間為1個月。
配套試劑
增值服務
相關產(chǎn)品
| 編號 | 適用物種：Mus musculus (Mouse，小鼠) | 應用(僅供研究使用，不用于臨床診斷！) |
| NPA082Mu01 | 層粘連蛋白(LN)天然蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAA082Mu01 | 層粘連蛋白(LN)多克隆抗體 | WB |
| MEA082Mu | 層粘連蛋白(LN)檢測試劑盒(酶聯(lián)免疫吸附試驗法,小樣本) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| SEA082Mu | 層粘連蛋白(LN)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| SCA082Mu | 層粘連蛋白(LN)檢測試劑盒(化學發(fā)光免疫分析法) | Chemiluminescent immunoassay for Antigen Detection. |
| LMA082Mu | 層粘連蛋白(LN)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
| KSA082Mu01 | 層粘連蛋白(LN)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法) | Main materials for "Do It (ELISA Kit) Yourself". |
參考文獻
| 雜志 | 參考文獻 |
| Stem Cell Research Therapy | Protein synthesis and secretion in human mesenchymal cells derived from bone marrow, adipose tissue and Wharton's jelly[Pubmed: 24739658] |
| PLoS One. | Mesenchymal stromal cell proliferation, gene expression and protein production in human platelet-rich plasma-supplemented media[Pubmed:Pmc4130592] |
| Clin Exp Metastasis. | Augmented expression of urokinase plasminogen activator and extracellular matrix proteins associates with multiple myeloma progression[Pubmed:24807734] |
| European Journal of Obstetrics & Gynecology and Reproductive Biology | Investigation?of?diagnostic?potentials?of?nine?different?biomarkers?in?endometriosis.[Pubmed:24813083] |
| Archives of Plastic Surgery | Comparative Analysis of the Extracellular Matrix Composition in Proliferating and Involuted Infantile Hemangiomas[PubMed: 26430624] |
| Canadian Journal of Physiology and Pharmacology | Modified Citrus Pectin stops progression of liver fibrosis by inhibiting galactin-3 and inducing apoptosis of stellate cells[Doi: Abs] |
| MOLECULAR AND CLINICAL ONCOLOGY | Clinical significance of serum laminin and typea??IV collagen levels in cutaneous melanoma patients[Pubmed:27330797] |
| Am J Transplant. | Key Matrix Proteins Within the Pancreatic Islet Basement Membrane Are Differentially Digested During Human Islet Isolation.[pubmed:27456745] |
| Asian Pacific Journal of Cancer Prevention | Modulatory Effects of Chemoradiation on Angiogenic Factors and Laminin in Cervical Cancer: Link with Treatment Response[pubmed:29172262] |
| Diabetes | Coordination Among Lipid Droplets, Peroxisomes and Mitochondria Regulates Energy Expenditure Through the CIDE-ATGL-PPARα Pathway in Adipocytes[Pubmed:29986925] |
| European Food Research and Technology | Evaluation of antifibrotic effects of coffee and cocoa extracts in rats with thioacetamide-induced fibrosis[10.1007:s00217-018-3119-z] |
| Experimental Lung Research | High amplitude stretching of ATII cells and fibroblasts results in profibrotic effects[Pubmed: 31290711] |
| Neurotherapeutics | Fabrication and Evaluation of a Xenogeneic Decellularized Nerve-Derived Material: Preclinical Studies of a New Strategy for Nerve Repair[Pubmed: 31758411] |
| Untersuchungen und semiquantitative Symmetrie-Analysen zur Extrazellul?rmatrix von Kalbs-Stimmlippen[] | |
| ARCHIV?EUROMEDICA | CLINICAL SIGNIFICANCE OF LAMININ AND ELASTINE LEVELS IN CHILDREN WITH UNDIFFERENTIATED CONNECTIVE TISSUE DISEASE[] |